anti total akt Search Results


total akt1  (Cell Signaling Technology Inc)
93
Cell Signaling Technology Inc total akt1
Total Akt1, supplied by Cell Signaling Technology Inc, used in various techniques. Bioz Stars score: 93/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/total akt1/product/Cell Signaling Technology Inc
Average 93 stars, based on 1 article reviews
total akt1 - by Bioz Stars, 2026-02
93/100 stars
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anti total protein kinase b akt  (Rockland Immunochemicals)
91
Rockland Immunochemicals anti total protein kinase b akt
Effects of wood creosote on diabetic obese mice treated with metformin. Wood creosote does not affect efficacy of metformin. Weight change (A) and blood glucose levels (fed condition) (B) were examined during the experiment in . Data are shown as means±SEM. No significant differences between the metformin group and the metformin with wood creosote (M+C) group were observed. Mice were fasted for 3 hours after the final administration of drugs. (C) One g/kg glucose was administrated via oral gavage. One to 2 µL blood was collected via tail vein at indicated time points, and blood glucose was measured by using GlucoSensor. (D) At day 4 of , mice were fasted for 2 hours and then injected intraperitoneally with 36 µg/kg insulin. Blood glucose levels are shown as % initial. At day 5 in , blood was collected and measured serum bile acid levels (E) and alanine aminotransferase (F) were measured. (G) Western blots were carried out using liver protein (50 µg) to detect levels of phospho-AMPK and <t>phoshopho-AKT.</t> AKT, Protein Kinase B; AMPK, AMP-activated kinase.
Anti Total Protein Kinase B Akt, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 91/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti total protein kinase b akt/product/Rockland Immunochemicals
Average 91 stars, based on 1 article reviews
anti total protein kinase b akt - by Bioz Stars, 2026-02
91/100 stars
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mouse anti-akt antibody  (Becton Dickinson)
90
Becton Dickinson mouse anti-akt antibody
a , b The effect of the two pharmacological inhibitors on the PI3K signalling cascade, as assessed by Western blot analysis, using phosphorylation of <t>Akt</t> and S6, as surrogate readouts for PI3K and mTOR activity, respectively. Analysed were G40 glioblastoma stem cells (left) and differentiated cells (right) in the presence of either GDC-0941 a or Rapamycin b . Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT <t>(Ser473)</t> <t>(#9271,</t> Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany). Of note, different exposure times were chosen between a and b to highlight the different effects of the two substances investigated. To facilitate a better comparison, relative protein phosphorylation was quantified using the ImageJ software packet (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/ , 1997–2011) and normalised to the control population. Red: phospho-Akt (Ser473)/Akt/ β-actin; blue: phospho-Akt (Thr308)/Akt/ β-actin; green: phospho-S6/S6/β-actin. c G40 SC (left) and DC (right) populations were incubated with either 0.6 μM GDC-0941 or 5 nM Rapamycin. Scratches were introduced 1 h after treatment initiation and from then semi-directional cell migration was examined after indicated time points by fixation of the cells and subsequent staining. Scale bars equal 500 μm. A representative result of at least two independent experiments is shown in a and b , while representative data of two independent experiments performed in triplicate are depicted in c
Mouse Anti Akt Antibody, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-akt antibody/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
mouse anti-akt antibody - by Bioz Stars, 2026-02
90/100 stars
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mouse anti-total akt  (EuroClone)
90
EuroClone mouse anti-total akt
a , b The effect of the two pharmacological inhibitors on the PI3K signalling cascade, as assessed by Western blot analysis, using phosphorylation of <t>Akt</t> and S6, as surrogate readouts for PI3K and mTOR activity, respectively. Analysed were G40 glioblastoma stem cells (left) and differentiated cells (right) in the presence of either GDC-0941 a or Rapamycin b . Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT <t>(Ser473)</t> <t>(#9271,</t> Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany). Of note, different exposure times were chosen between a and b to highlight the different effects of the two substances investigated. To facilitate a better comparison, relative protein phosphorylation was quantified using the ImageJ software packet (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/ , 1997–2011) and normalised to the control population. Red: phospho-Akt (Ser473)/Akt/ β-actin; blue: phospho-Akt (Thr308)/Akt/ β-actin; green: phospho-S6/S6/β-actin. c G40 SC (left) and DC (right) populations were incubated with either 0.6 μM GDC-0941 or 5 nM Rapamycin. Scratches were introduced 1 h after treatment initiation and from then semi-directional cell migration was examined after indicated time points by fixation of the cells and subsequent staining. Scale bars equal 500 μm. A representative result of at least two independent experiments is shown in a and b , while representative data of two independent experiments performed in triplicate are depicted in c
Mouse Anti Total Akt, supplied by EuroClone, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/mouse anti-total akt/product/EuroClone
Average 90 stars, based on 1 article reviews
mouse anti-total akt - by Bioz Stars, 2026-02
90/100 stars
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r-pe-labeled anti-total akt  (Becton Dickinson)
90
Becton Dickinson r-pe-labeled anti-total akt
a , b The effect of the two pharmacological inhibitors on the PI3K signalling cascade, as assessed by Western blot analysis, using phosphorylation of <t>Akt</t> and S6, as surrogate readouts for PI3K and mTOR activity, respectively. Analysed were G40 glioblastoma stem cells (left) and differentiated cells (right) in the presence of either GDC-0941 a or Rapamycin b . Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT <t>(Ser473)</t> <t>(#9271,</t> Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany). Of note, different exposure times were chosen between a and b to highlight the different effects of the two substances investigated. To facilitate a better comparison, relative protein phosphorylation was quantified using the ImageJ software packet (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/ , 1997–2011) and normalised to the control population. Red: phospho-Akt (Ser473)/Akt/ β-actin; blue: phospho-Akt (Thr308)/Akt/ β-actin; green: phospho-S6/S6/β-actin. c G40 SC (left) and DC (right) populations were incubated with either 0.6 μM GDC-0941 or 5 nM Rapamycin. Scratches were introduced 1 h after treatment initiation and from then semi-directional cell migration was examined after indicated time points by fixation of the cells and subsequent staining. Scale bars equal 500 μm. A representative result of at least two independent experiments is shown in a and b , while representative data of two independent experiments performed in triplicate are depicted in c
R Pe Labeled Anti Total Akt, supplied by Becton Dickinson, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/r-pe-labeled anti-total akt/product/Becton Dickinson
Average 90 stars, based on 1 article reviews
r-pe-labeled anti-total akt - by Bioz Stars, 2026-02
90/100 stars
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anti-mouse (akt) antibodies for total protein  (Rockland Immunochemicals)
90
Rockland Immunochemicals anti-mouse (akt) antibodies for total protein
a , b The effect of the two pharmacological inhibitors on the PI3K signalling cascade, as assessed by Western blot analysis, using phosphorylation of <t>Akt</t> and S6, as surrogate readouts for PI3K and mTOR activity, respectively. Analysed were G40 glioblastoma stem cells (left) and differentiated cells (right) in the presence of either GDC-0941 a or Rapamycin b . Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT <t>(Ser473)</t> <t>(#9271,</t> Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany). Of note, different exposure times were chosen between a and b to highlight the different effects of the two substances investigated. To facilitate a better comparison, relative protein phosphorylation was quantified using the ImageJ software packet (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/ , 1997–2011) and normalised to the control population. Red: phospho-Akt (Ser473)/Akt/ β-actin; blue: phospho-Akt (Thr308)/Akt/ β-actin; green: phospho-S6/S6/β-actin. c G40 SC (left) and DC (right) populations were incubated with either 0.6 μM GDC-0941 or 5 nM Rapamycin. Scratches were introduced 1 h after treatment initiation and from then semi-directional cell migration was examined after indicated time points by fixation of the cells and subsequent staining. Scale bars equal 500 μm. A representative result of at least two independent experiments is shown in a and b , while representative data of two independent experiments performed in triplicate are depicted in c
Anti Mouse (Akt) Antibodies For Total Protein, supplied by Rockland Immunochemicals, used in various techniques. Bioz Stars score: 90/100, based on 1 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
https://www.bioz.com/result/anti-mouse (akt) antibodies for total protein/product/Rockland Immunochemicals
Average 90 stars, based on 1 article reviews
anti-mouse (akt) antibodies for total protein - by Bioz Stars, 2026-02
90/100 stars
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Image Search Results


Effects of wood creosote on diabetic obese mice treated with metformin. Wood creosote does not affect efficacy of metformin. Weight change (A) and blood glucose levels (fed condition) (B) were examined during the experiment in . Data are shown as means±SEM. No significant differences between the metformin group and the metformin with wood creosote (M+C) group were observed. Mice were fasted for 3 hours after the final administration of drugs. (C) One g/kg glucose was administrated via oral gavage. One to 2 µL blood was collected via tail vein at indicated time points, and blood glucose was measured by using GlucoSensor. (D) At day 4 of , mice were fasted for 2 hours and then injected intraperitoneally with 36 µg/kg insulin. Blood glucose levels are shown as % initial. At day 5 in , blood was collected and measured serum bile acid levels (E) and alanine aminotransferase (F) were measured. (G) Western blots were carried out using liver protein (50 µg) to detect levels of phospho-AMPK and phoshopho-AKT. AKT, Protein Kinase B; AMPK, AMP-activated kinase.

Journal: BMJ Open Diabetes Research & Care

Article Title: Mouse model of metformin-induced diarrhea

doi: 10.1136/bmjdrc-2019-000898

Figure Lengend Snippet: Effects of wood creosote on diabetic obese mice treated with metformin. Wood creosote does not affect efficacy of metformin. Weight change (A) and blood glucose levels (fed condition) (B) were examined during the experiment in . Data are shown as means±SEM. No significant differences between the metformin group and the metformin with wood creosote (M+C) group were observed. Mice were fasted for 3 hours after the final administration of drugs. (C) One g/kg glucose was administrated via oral gavage. One to 2 µL blood was collected via tail vein at indicated time points, and blood glucose was measured by using GlucoSensor. (D) At day 4 of , mice were fasted for 2 hours and then injected intraperitoneally with 36 µg/kg insulin. Blood glucose levels are shown as % initial. At day 5 in , blood was collected and measured serum bile acid levels (E) and alanine aminotransferase (F) were measured. (G) Western blots were carried out using liver protein (50 µg) to detect levels of phospho-AMPK and phoshopho-AKT. AKT, Protein Kinase B; AMPK, AMP-activated kinase.

Article Snippet: Antibodies are as follows: Anti-glyceraldehyde 3-phosphate dehydrogenase (GAPDH) (1E6D9, Proteintech, Rosemont, Illinois, USA), anti-AMPK (ABV10739, ABGENT, San Diego, California, USA), anti-phospho AMPK (pT172) (40H9, Cell Signaling Technology, Danvers, Massachusetts, USA), anti-total Protein Kinase B (AKT) (200–401 N98, Rockland, Limerick, Pennsylvania, USA), and anti-phosho AKT (pS473) (D9E, Cell Signaling Technology).

Techniques: Injection, Western Blot

a , b The effect of the two pharmacological inhibitors on the PI3K signalling cascade, as assessed by Western blot analysis, using phosphorylation of Akt and S6, as surrogate readouts for PI3K and mTOR activity, respectively. Analysed were G40 glioblastoma stem cells (left) and differentiated cells (right) in the presence of either GDC-0941 a or Rapamycin b . Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT (Ser473) (#9271, Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany). Of note, different exposure times were chosen between a and b to highlight the different effects of the two substances investigated. To facilitate a better comparison, relative protein phosphorylation was quantified using the ImageJ software packet (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/ , 1997–2011) and normalised to the control population. Red: phospho-Akt (Ser473)/Akt/ β-actin; blue: phospho-Akt (Thr308)/Akt/ β-actin; green: phospho-S6/S6/β-actin. c G40 SC (left) and DC (right) populations were incubated with either 0.6 μM GDC-0941 or 5 nM Rapamycin. Scratches were introduced 1 h after treatment initiation and from then semi-directional cell migration was examined after indicated time points by fixation of the cells and subsequent staining. Scale bars equal 500 μm. A representative result of at least two independent experiments is shown in a and b , while representative data of two independent experiments performed in triplicate are depicted in c

Journal: Oncogenesis

Article Title: The effects of PI3K-mediated signalling on glioblastoma cell behaviour

doi: 10.1038/s41389-017-0004-8

Figure Lengend Snippet: a , b The effect of the two pharmacological inhibitors on the PI3K signalling cascade, as assessed by Western blot analysis, using phosphorylation of Akt and S6, as surrogate readouts for PI3K and mTOR activity, respectively. Analysed were G40 glioblastoma stem cells (left) and differentiated cells (right) in the presence of either GDC-0941 a or Rapamycin b . Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT (Ser473) (#9271, Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany). Of note, different exposure times were chosen between a and b to highlight the different effects of the two substances investigated. To facilitate a better comparison, relative protein phosphorylation was quantified using the ImageJ software packet (Rasband, W.S., ImageJ, U. S. National Institutes of Health, Bethesda, MD, USA, http://imagej.nih.gov/ij/ , 1997–2011) and normalised to the control population. Red: phospho-Akt (Ser473)/Akt/ β-actin; blue: phospho-Akt (Thr308)/Akt/ β-actin; green: phospho-S6/S6/β-actin. c G40 SC (left) and DC (right) populations were incubated with either 0.6 μM GDC-0941 or 5 nM Rapamycin. Scratches were introduced 1 h after treatment initiation and from then semi-directional cell migration was examined after indicated time points by fixation of the cells and subsequent staining. Scale bars equal 500 μm. A representative result of at least two independent experiments is shown in a and b , while representative data of two independent experiments performed in triplicate are depicted in c

Article Snippet: Western blot analysis was performed as previously described , and following antibodies were used: rabbit anti-phospho-AKT (Ser473) (#9271, Cell Signaling, Leiden, The Netherlands), rabbit anti-phospho-AKT (Thr308) (#9275, Cell Signaling), mouse anti-AKT antibody (#610860, BD Bioscience), rabbit anti-phospho-S6 ribosomal protein (Ser235/236) (#2211, Cell Signaling), rabbit anti-S6 ribosomal protein (#2317, Cell Signaling), β-actin (#A5441, Sigma-Aldrich) followed by goat anti-mouse IgG or goat anti-rabbit IgG-conjugated to horseradish peroxidase (Santa Cruz Biotechnology, Heidelberg, Germany).

Techniques: Western Blot, Activity Assay, Software, Incubation, Migration, Staining